C1821
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 | Immunoblotting Madin Darby Bovine Kidney (MDBK) extract was separated on SDS-PAGE and probed with Monoclonal AB to PAN Cadherin (MO) CL: CH-19 (PREPK) (Cat. No. C1821). The antibody was developed with HRP APA Mouse Fab ads/HIgG conjugate (Cat. No. A9917) and a NBT/BCIP substrate. Antibody dilution 1/2,000 |
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C1821
Sigma
Monoclonal Anti-Pan Cadherin antibody produced in mouse
clone CH-19, ascites fluid
| Synonym: | Monoclonal Anti-Pan Cadherin |
Description
| Immunogen | synthetic peptide corresponding to the C-terminal amino acids of chicken N-Cadherin with an extra N-terminal lysine residue (24 amino acids) coupled to KLH. |
| Specificity | Detection of members of the cadherin family or genetically engineered proteins containing the C-terminal cadherin tail, stains adherens type cell-cell junctions regardless of their cadherin type. |
Properties
| antibody form | ascites fluid |
| clone | CH-19 (monoclonal) |
| mol wt | antigen mol wt 135 kDa |
| contains | 15 mM sodium azide |
| species reactivity | hamster, mouse, rat, bovine, feline, chicken, human, canine, sheep, frog, guinea pig, pig, snake, Psammomys (sand rat), goat, rabbit |
| application(s) | immunoblotting: suitable |
| immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested animal heart sections | |
| indirect immunofluorescence: 1:500 using cultured MDBK cells | |
| microarray: suitable | |
| isotype | IgG1 |
| Gene Information | human ... CDH1(999), CDH2(1000), CDH3(1001) |
| rat ... Cdh1(83502), Cdh2(83501), Cdh3(116777) | |
| mouse ... Cdh1(12550), Cdh2(12558), Cdh3(12560) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | NWG |
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References
| reference | Takeichi, M. Development 102, 639, (1988) Abstract |
| Charlton, C.A., et al., Fusion Competence of Myoblasts Rendered Genetically Null for N-Cadherin in Culture J. Cell Biol. 138, 331-336, (1997) Abstract | |
| Shani, G., et al., GRP78 and Cripto Form a Complex at the Cell Surface and Collaborate To Inhibit Transforming Growth Factor β Signaling and Enhance Cell Growth Mol. Cell. Biol. 28, 666-677, (2008) Abstract | |
| Riveline, D., et al., Membrane and acto-myosin tension promote clustering of adhesion proteins Proc. Natl. Acad. Sci. U. S. A. 101, 2229-2234, (2004) Abstract |
